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This edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes. This book is divided in 8 sections offering a selection of technologies widely used in the characterization of protein and protein-nucleic acid complexes for different purposes and at different scales. From native electrophoresis methods, that are accessible to any reasonably well-equipped laboratory, to the sophisticated setup required for structure determination by cryo-electron microscopy or X-ray crystallography, this book contains a wide variety of clearly explained analytic and preparative approaches, connected with the production techniques developed on the previous volume. The readers will find an integral connection between this book and the first volume, which ensures a comprehensive and updated discussion of the main topics of the discipline. Taken together, these volumes constitute a cohesive and authoritative source of the research on multicomponent macromolecular complexes. In here, we focus on characterization of protein complexes in the broadest sense, which is not typically covered in other sources. Moreover, all chapters are carefully written by world renowned scientists and active researchers, making this volume ideal, not only as a reference source, but also as a companion book for the daily laboratory work. This book is aimed for a wide range of scientists, from science students to experienced researchers, working on protein and protein-nucleic acid complexes, who need a thorough understanding of protein production and complex characterization. .

Life sciences. --- Life Sciences.

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Life sciences.

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Life sciences

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This book compiles research on Enterobacterales characterization concerning the presence of genes associated with virulence (adhesins; surface cellulose structures and curli; siderophores, e.g., enterobactin, aerobactin, and yersiniabactin; protectines; invasins or toxins), and, furthermore, bacterial-biofilm-associated phenotypes. Although not directly involved in pathogenicity, the acquisition of multiple antibiotic resistances strongly supports the success of opportunistic Enterobacterales pathogens in invasion, survival, and spread and markedly complicates the treatment of infections. Not only pathogens, but also commensal bacteria, considered harmless and part of the normal microbiota, are exposed to selection pressure and can be a reservoir of mobile genetic elements carrying antibiotic resistance genes. Therefore, the occurrence of drug-resistant bacteria within a commensal population and the possibility to exchange genetic material through horizontal gene transfer may represent a major health concern.

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The cultivation and harvesting of sugar beets (roots and haulm) is one of the most labour and energy consuming work processes in the agricultural industry. The improvement of the qualitative indicators of the sugar beet harvesting process as the final operation in the work flow of its production represents a multi-faceted research-and-engineering problem, which is to be solved basing on the search for new implement design concepts and overall beet harvester design layouts, the thorough theoretical substantiation of their design and process parameters, the experimental verification of the completed theoretical research with the ultimate objective of the analysis and synthesis of their rational parameters. Theoretical research must play a fundamental role in the mechanical and technological substantiation of the root lifting process. It must be used as the basis for developing rational kinematic and dynamic operation conditions in order to achieve the required quality of the performed work process as well as the streamlined energy consumption. The book can be considered as one of the first solid analytical studies of the process of vibrational digging of sugar beet roots. It offers the analysis of up-to-date engineering solutions of vibrational digging tools and experimental investigations of the first vibrational lifters, the equation of oscillating motions of digging shares.

Life sciences.